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[1]张佑红,杨文,危威,等.杆状病毒在气升式反应器中感染策略优化[J].武汉工程大学学报,2012,(06):22-26.[doi:103969/jissn16742869201206006]
　ZHANG You\|hong,YANG Wen,WEI Wei,et al.Optimization of infection strategy for baculovirus in airlift reactor[J].Journal of Wuhan Institute of Technology,2012,(06):22-26.[doi:103969/jissn16742869201206006]
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杆状病毒在气升式反应器中感染策略优化(/HTML)
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《武汉工程大学学报》[ISSN:1674-2869/CN:42-1779/TQ]

卷:
期数:
2012年06期
页码:
22-26
栏目:
化学与化学工程
出版日期:
2012-06-30

文章信息/Info

Title:
Optimization of infection strategy for baculovirus in airlift reactor
文章编号:
16742869(2012)06002205
作者:
张佑红; 杨文; 危威; 徐智鹏; 苏腾甲
武汉工程大学化工与制药学院，绿色化工过程省部共建教育部重点实验室，湖北 武汉 430074
Author(s):
ZHANG You\|hong; YANG Wen; WEI Wei; XU Zhi\|peng; SU Teng\|jia
Key Laboratory for Green Chemical Process of Ministry of Education,
School of Chemical Engineering and Pharmacy,Wuhan Institute of Technology,Wuhan 430074,China
关键词:
杆状病毒; 昆虫细胞; 感染策略优化
Keywords:
baculovirus;  insect cell;  infection strategy
分类号:
Q819
DOI:
103969/jissn16742869201206006
文献标志码:
A
摘要:
为了得到杆状病毒感染悬浮昆虫细胞最佳条件，利用正交实验探讨了气升式反应器中感染复数（MOI）、感染时间（TOI）、细胞初始浓度（ICD）及体系中的溶氧值（DO）的相互作用关系以及对包涵体病毒（OV）和非包涵体病毒（BV）产量的影响.结果表明：最佳感染条件为MOI为0.1，TOI为细胞处于对数生长期晚期，ICD为2×105 cell·mL-1，DO为10%，最大BV病毒滴度为7.943×106 TCID50·mL-1，最大OV病毒滴度为1.68×106 OVs·mL-1.
Abstract:
To get the best conditions for the baculovirus infection of suspension insect cells,an orthogonal design was used to select the optimum conditions,in which the effects of multiplicity of infection (MOI),time of infection(TOI),initial cell density(ICD） and dissolved oxygen (DO）on the budded virus (BV）titer and occluded virus(OV）number were evaluated in the HzAM1 insect cell\|recombinant baculovirus system at a low MOI in airlift reactor. The results show that the best conditions for the infection are MOI of 0.1,initial cell density of 2×105 cell·mL-1 ,infection at the late exponential phase and DO of 10%,and a maximum BV concentration of 7.943×106 TCID50·mL-1 is obtained,a maximum OV concentration of 1.68×106 OVs·mL-1 is obtained.

参考文献/References:

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［4］张佑红,王华林,陈燕,等. 适合棉铃虫细胞HzAm1生长的培养基筛选及低血清驯化［J］. 生物工程学报,2006,22: 686-688.
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［12］徐鹏，张佑红，杨益,等.流式细胞术快速检测杆状病毒滴度［J］.武汉工程大学学报,2010(1):57-60.

相似文献/References:

[1]徐鹏,张佑红*,杨益,等.流式细胞术快速检测杆状病毒滴度[J].武汉工程大学学报,2010,(01):57.
　XU Peng,YANG Yi,PENG Ji ming,et al.Rapid titration of baculovirus by flow cytometry[J].Journal of Wuhan Institute of Technology,2010,(06):57.

备注/Memo

备注/Memo:
收稿日期：20120227基金项目：国家自然科学基金项目（20876120）；武汉市科技攻关项目（20052002044）作者简介：张佑红（1964-），男，湖南浏阳人，教授，博士，博士研究生导师.研究方向：细胞生物学，分子生物学.
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