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[1]黄坤,郭庆东,谢芳,等.高效液相色谱法测定奥氮平的含量[J].武汉工程大学学报,2012,(4):11-14.
　HUANG Kun,GUO Qing\|dong,XIE Fang,et al.Determination of olanzapine content by reversed phase high performance liquid[J].Journal of Wuhan Institute of Technology,2012,(4):11-14.
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高效液相色谱法测定奥氮平的含量
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《武汉工程大学学报》[ISSN:1674-2869/CN:42-1779/TQ]

卷:
期数:
2012年4期
页码:
11-14
栏目:
化学与化学工程
出版日期:
2012-05-30

文章信息/Info

Title:
Determination of olanzapine content by reversed
phase high performance liquid
文章编号:
16742869(2012)04001104
作者:
黄坤1; 2; 郭庆东2; 谢芳1; 2; 王增明1; 2; 郭嘉1; 梅兴国2*
1.武汉工程大学绿色化工过程教育部重点实验室，湖北 武汉 430074；
2.军事医学科学院毒物药物研究所，北京 100850
Author(s):
HUANG Kun1; 2;  GUO Qing\|dong2;  XIE Fang1; 2;  WANG Zeng\|ming1; 2;  GUO Jia1;  MEI Xing\|guo2
1.Key Laboratory for Green Chemical Process of Ministry of Eduction,Wuhan Institute of Technology,Wuhan 430074,China;
2. Institute of Pharmacology and Toxicology, Academy of Military Medical Sciences, Beijing 100850, China
关键词:
高效液相色谱法; 奥氮平; 口崩片; 含量测定
Keywords:
high performance liquid chromatography;  olanzapine;  orally disintegrating tablets;  quantitative determination
分类号:
R917
DOI:
-
文献标志码:
AAdoi:103969/jissn16742869201204003
摘要:
为了建立一种高效液相色谱法测定奥氮平及其口崩片的含量，采用Venusil ASB C18色谱柱（4.6 mm×150 mm，5 μm）进行分析，流动相为缓冲液（pH=2.5）∶乙腈=45∶55（体积比）；检测波长为259 nm；柱温为25 ℃；流速为1.5 mL·min-1；进样量为20 μL. 结果表明：奥氮平在20.33～203.30 μg·mL-1范围内线性关系良好（r=0.999 8），平均回收率为100.12%（RSD=0.33%），检测限为1 ng；该方法灵敏快速、重复性好、专属性强，可用于非经典抗精神病药奥氮平及其口崩片的含量测定.
Abstract:
To establish a high performance liquid chromatography method for determining the content of olanzapine and in orally disintegrating tablets, the best HPLC conditions were set as follows: a Venusil ASB C18(4.6 mm×150 mm, 5 μm)column, mobile phase of buffer solution (pH=2.5)-acetonitrile(45∶55), ultraviolet absorption detector of 259 nm, column temperature of 25 ℃, flow rate of 1.5 mL·min-1, injection volume of 20 μL. The results show that the linearity of olanzapine is in the range of 20.33 to 203.30 μg·mL-1(r=0.9998), the average recovery is 100.12% with relative standard deviation(RSD) of 0.33%, the limit of detection(LOD) is 1 ng. The method is sensitive, quick, repeatable, and thus it can be provided for determining the content of olanzapine and in orally disintegrating tablets.

参考文献/References:

［1］李娜, 许秀峰. 奥氮平的药理学和临床应用［J］. 云南医药, 2004，25 (6）: 521-522.
［2］崔炳喜. 奥氮平——新型抗精神病药［J］. 天津药学, 1999, 11 (4）: 13-14.
［3］温中京, 戴建锋. 口腔崩解片的研究进展［J］. 海峡药学, 2010 , 22 (4）: 22-24.
［4］寻知元. 药物干预与改善分裂症患者的依从性［J］. 国际精神病学杂志, 2007, 34 (1）: 39-42.
［5］Berna M, Ackermann B, Ruterbories K, et al. Determination of olanzapine in human blood by liquid chromatography-tandem mass spectrometry［J］. Journal of Chromatography B, 2002, 767 (1）: 163-168.
［6］D’Arrigo C, Migliardi G, Santoro V, et al. Determination of olanzapine in human plasma by reversed-phase high-performance liquid chromatography with ultraviolet detection［J］. Ther Drug Monit, 2006, 28 (3）: 388-393.
［7］季慧芳，谢林，王建，等. 奥氮平血药浓度的测定及其人体药代动力学［J］. 中国药科大学学报, 2002, 33 (5）: 397-400.
［8］唐亚芳，宋伟明，吴柳松. 反相高效液相色谱法测定人血清中奥氮平浓度［J］. 临床医药， 2007, 16  (21）: 46-47.
［9］Raggi M A, Casamenti G, Mandrioli R, et al. Quantitation of olanzapine in tablets by HPLC, CZE, derivative spectrometry and linear voltammetry［J］. Journal of Pharmaceutical and Biomedical Analysis, 2000, 23 (6）: 973-981.
［10］Krishnaiah C, Vishnu M M, Kumar R, et al. Development of a stability\|indicating UPLC method for determining olanzapine and its associated degradation products present in active pharmaceutical ingredients and pharmaceutical dosage forms［J］. Journal of Pharmaceutical and Biomedical Analysis, 2011, 54 (4）: 667-673.

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