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[1]刘永琼,李金,张爽爽,等.泡腾片中硫胺素含量的高效液相色谱法测定[J].武汉工程大学学报,2014,(06):1-4.[doi:103969/jissn16742869201406001]
　LIU Yong qiong,LI Jin,ZHANG Shuang shuang,et al.Determination of vitamin B1 in effervescent tablet byhigh performance liquid chromatography[J].Journal of Wuhan Institute of Technology,2014,(06):1-4.[doi:103969/jissn16742869201406001]
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泡腾片中硫胺素含量的高效液相色谱法测定(/HTML)
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《武汉工程大学学报》[ISSN:1674-2869/CN:42-1779/TQ]

卷:
期数:
2014年06期
页码:
1-4
栏目:
化学与化学工程
出版日期:
2014-06-30

文章信息/Info

Title:
Determination of vitamin B1 in effervescent tablet byhigh performance liquid chromatography
文章编号:
16742869(2014)06000104
作者:
刘永琼1; 2; 李金1; 2; 张爽爽1; 2; 肖芳3; 余佩1; 2
1.武汉工程大学化工与制药学院，湖北 武汉 430074；2.湖北省新型反应器与绿色化学工艺重点实验室(武汉工程大学)，湖北 武汉 430074；3.国家食品药品监督管理总局行政受理服务中心，北京 100036
Author(s):
LIU Yongqiong1; 2; LI Jin1; 2; ZHANG Shuangshuang1; 2; XIAO Fang3; YU Pei1; 2
1.School of Chemical Engineering and Pharmacy，Wuhan Institute of Technology，Wuhan 430074，China；2.Hubei Key Laboratory of Novel Chemical Reactor & Green Chemical Technology(Wuhan Institute of Technology)，Wuhan 430074，China；3.State Food and Drug Administration accepted the administrative service center,Beijing 100036,China
关键词:
高效液相色谱法; 泡腾片; 维生素B1
Keywords:
high performance liquid chromatography; effervescent tablet; vitamin B1
分类号:
TQ460.72
DOI:
103969/jissn16742869201406001
文献标志码:
A
摘要:
为了快速、精确测定泡腾片中硫胺素（维生素B1）的含量，采用了高效液相色谱的分析方法，以Dionex C18(粒径5 μm，4.6 mm×250 mm)为分离柱，0.05 mol/L 磷酸二氢钾 (pH=7)∶甲醇=90∶10的溶液为流动相，流速为1.0 mL/min，检测波长238 nm，柱温为25 ℃.结果表明，泡腾片中维生素B1的质量浓度在8～48 mg/L范围内与峰面积呈良好线性关系 (R2=0.999 1，n=11)；回收率在100%~105%之间，相对标准偏差 (RSD) 小于5%；最低检出限为0.4 mg/L；且维生素B1与其降解产物分离度良好，溶剂与辅料不干扰样品的测定.该方法精密度良好，准确度高，可作为控制产品质量的方法.
Abstract:
To determine the content of effervescent tablets of vitamin B1 quickly and accurately,we adopted the high performance liquid chromatography (HPLC) method,using Dionex C18(4.6 mm×250 mm,5 μm) as column,methanol0.05 mol/L Potassium dihydrogen(pH=7) (90∶10) as phosphate at flow rate of 1 mL/min,detection wavelength of 238 nm and 25 ℃.The results show that calibration curve of peak area and concentration is linear in the range of 848 mg/L;the average recovery is between 100 and 105%,and the relative standard deviation is less than 5% (R2=0.999 1，n=11);the lowest detection limit is 0.4 mg/L.In addition,the separation degree of Vitamin B1 and its degradation products is good.Solvent and accessories have not an effect on the determination of samples.The HPLC method is simple and accurate to control the quality of products.

参考文献/References:

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备注/Memo

备注/Memo:
收稿日期：20140415基金项目：2012年地方高校国家级大学生创新创业训练计划项目（教高司函\[2013\]8号：201210490013）作者简介：刘永琼（1953），女，湖北枣阳人，教授，硕士研究生导师.研究方向：药物合成、药物制剂与天然药物.
更新日期/Last Update: 2014-07-29