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[1]杨浩,宋涛,赵慧平,等.大肠杆菌在原子力显微镜下的观测及其形貌[J].武汉工程大学学报,2012,(12):8-12.[doi:103969/jissn16742869201212003]
　YANG Hao,SONG Tao,ZHAO Hui ping,et al.Imaging and morphology of Escherichia coli using atomic force microscopy[J].Journal of Wuhan Institute of Technology,2012,(12):8-12.[doi:103969/jissn16742869201212003]
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大肠杆菌在原子力显微镜下的观测及其形貌(/HTML)
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《武汉工程大学学报》[ISSN:1674-2869/CN:42-1779/TQ]

卷:
期数:
2012年12期
页码:
8-12
栏目:
化学与化学工程
出版日期:
2013-01-11

文章信息/Info

Title:
Imaging and morphology of Escherichia coli using atomic force microscopy
文章编号:
16742869(2012)12000805
作者:
杨浩; 宋涛; 赵慧平; 吕中; 陈嵘*
武汉工程大学绿色化工过程教育部重点实验室，湖北省新型反应器与
绿色化学工艺重点实验室，湖北　武汉　430074
Author(s):
YANG Hao;  SONG Tao; ZHAO Huiping;  LU Zhong;  CHEN Rong
Key Laboratory for Green Chemical Process of Ministry of Education and Hubei Key Laboratory of
Novel Reactor and Green Chemical Technology，Wuhan Institute of Technology，Wuhan 430074，China
关键词:
原子力显微镜; 大肠杆菌; 扫描参数; 表面微观结构
Keywords:
atomic force microscopy;  Escherichia coli;  scanning parameter;  surface microstructure
分类号:
Q248
DOI:
103969/jissn16742869201212003
文献标志码:
A
摘要:
对细菌形貌的观测是研究细菌行为的基础，为了准确观测细菌的表面形貌，实验以大肠杆菌为研究对象，采用轻敲模式，在空气中利用原子力显微镜（AFM）对大肠杆菌进行扫描.实验优化了AFM的扫描参数以获得高质量的图像，并定量分析了大肠杆菌的表面形貌特征.结果表明，分辨率、扫描频率、setpoint值和I/P值对AFM图像的清晰度具有重要影响，参数选择不当都会使图像模糊或失真.实验得到的最佳扫描参数为：分辨率256，扫描频率2 Hz，setpoint值1.645 V，I/P值0.3/0.4.所扫描的大肠杆菌长度为(985.2±76.8)nm，宽度为(751.5±74.7)nm，高度为(168.4±39.3)nm.研究表明，AFM既可以定性表征微生物细胞的表面结构特征，也可以定量分析细胞的形貌参数，这为深入研究微生物的生理活动和抗菌剂的作用机理提供了重要的依据和手段.
Abstract:
The observation of the morphology of bacterial cell is fundamental for understanding the behavior of bacteria. To facilitate accurate observation, Escherichia coli, as a model bacterium, was imaged by atomic force microscopy (AFM) under trapping mode in air. AFM scanning parameters were optimized to obtain the high quality of the AFM image. In addition, the profiles of the bacterial morphology were quantitatively analyzed. The results show that the resolution, scanning frequency, setpoint and I/P gains have important effects on the quality of the AFM image, and the image would be obscure and fuzzy at inappropriate conditions. In this experiment, the optimal parameters are as follow: resolution 256, scanning frequency 2 Hz, setpoint 1.645 V, I/P gain 0.3/0.4. The length, width and height of Escherichia coli are (985.2±76.8)nm, (751.5±74.7)nm and (52.8±10.2)nm, respectively. This research reveals that AFM could not only analyze the surface features of microbe qualitatively, but also determine the size of the microbial cell quantitatively, which provide significant evidence and method to study the physiological activity of microbe and antibacterial mechanism of antibacterial agent.

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备注/Memo

备注/Memo:
收稿日期：20121029基金项目：国家自然科学基金项目（21201135）；湖北省教育厅科学技术研究项目(Q20121502,D20111510)；武汉市科技局对外科技合作与交流计划（201231234465,20117103419）；武汉工程大学环境与化工清洁生产实验教学示范中心创新型实验项目作者简介：杨浩（1981），男，湖北武汉人，讲师，博士．研究方向：功能界面材料.
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