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[1]朱雄伟,熊瑶,苏腾甲.降解多聚半乳糖醛酸菌株的诱变[J].武汉工程大学学报,2012,(11):14-17.[doi:103969/jissn16742869201211004]
　ZHU Xiong wei,XIONG Yao,SU Teng jia.Mutation of polygalacturonic acid degrading strain[J].Journal of Wuhan Institute of Technology,2012,(11):14-17.[doi:103969/jissn16742869201211004]
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降解多聚半乳糖醛酸菌株的诱变(/HTML)
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《武汉工程大学学报》[ISSN:1674-2869/CN:42-1779/TQ]

卷:
期数:
2012年11期
页码:
14-17
栏目:
化学与化学工程
出版日期:
2012-12-10

文章信息/Info

Title:
Mutation of polygalacturonic acid degrading strain
文章编号:
16742869(2012)11001404
作者:
朱雄伟; 熊瑶; 苏腾甲
武汉工程大学化工与制药学院，绿色化工过程省部共建教育部重点实验室，湖北 武汉 430074
Author(s):
ZHU Xiongwei;  XIONG Yao;  SU Tengjia
Key Laboratory for Green Chemical Process of Ministry of Education, School of Chemical Engineering and Pharmacy,
Wuhan Institute of Technology, Wuhan 430074, China
关键词:
诱变; 紫外; 硫酸二乙脂; 叠氮钠; 马来酰肼
Keywords:
mutation;  ultraviolet;  diethyl sulfate;  sodium azide;  maleic hydrazid
分类号:
Q933
DOI:
103969/jissn16742869201211004
文献标志码:
A
摘要:
从食品工业的废水池中筛选分离得到一株能有效降解多聚半乳糖醛酸的菌株，通过测定其16 S rRNA的序列，证明该菌株属细菌类，命名为HDYM02.为了使分离得到的原始菌株降解多聚半乳糖醛酸的能力达到实际应用的要求，运用硫酸二乙酯、叠氮钠、马来酰肼及紫外对降解多聚半乳糖醛酸生产菌菌株HDYM02进行诱变，使用刚果红染色法对诱变得到菌株进行筛选，并检测突变菌株对多聚半乳糖醛酸降解能力，选育出高效降解多聚半乳糖醛酸的菌株.结果表明：出发菌株经过叠氮钠诱变30 min时，诱变得到的菌株降解效率高，是出发菌株的1.66倍，达到实际应用的要求.
Abstract:
One strain degrading polygalacturonic acid effectively was screened and separated from wastewater reservoir of food industry, the strain was proved to be bacterium by determining its 16S rRNA sequence, which was nominated HDYM02. To make the originally separated strain ability of degrading polygalacturonic acid reach the requirement of practical application, diethyl sulfate, sodium azide, maleic hydrazide and ultraviolet were used to mutate the strain HDYM02 which degrades polygalacturonic acid. Congo red decoration method was used to select mutant strain, then the degradation ability of polygalacturonic acid was detected. And the highly efficient strain of degrading polygalacturonic acid was bred. The results show that when the original strain is mutated for 30 min by sodium azide, the mutant strain has a higher degradation ability, which is 1.66 times of the original strain’s, and can reach the requirement of practical application.

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备注/Memo

备注/Memo:
收稿日期：20120703作者简介：朱雄伟（1973），男，湖北咸宁人，讲师，博士研究生.研究方向：微生物培养.
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