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[1]苏腾甲,朱雄伟,张佑红*,等.果胶酶生产菌株的分离及其产酶条件优化[J].武汉工程大学学报,2012,(4):15-18.
　SU Teng\|jia,ZHU Xiong\|wei,ZHANG You\|hong,et al.Separation of one pectinase strain and optimization for it’s production of enzymes[J].Journal of Wuhan Institute of Technology,2012,(4):15-18.
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果胶酶生产菌株的分离及其产酶条件优化(/HTML)
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《武汉工程大学学报》[ISSN:1674-2869/CN:42-1779/TQ]

卷:
期数:
2012年4期
页码:
15-18
栏目:
化学与化学工程
出版日期:
2012-05-30

文章信息/Info

Title:
Separation of one pectinase strain and optimization for
it’s production of enzymes
文章编号:
16742869(2012)04001504
作者:
苏腾甲; 朱雄伟; 张佑红*; 刘芬; 刘婷婷; 徐智鹏; 李卫朋
武汉工程大学化工与制药学院，绿色化工过程省部共建教育部重点实验室，湖北 武汉 430074
Author(s):
SU Teng\|jia;  ZHU Xiong\|wei; ZHANG You\|hong;  LIU Fen; LIU Ting\|ting; XU Zhi\|peng; LI Wei\|peng
Key Laboratory for Green Chemical Process of Ministry of Education, School of Chemical Engineering and
Pharmacy, Wuhan Institute of Technology, Wuhan 430074, China
关键词:
果胶酶生产菌株; 纯化; 发酵; 产酶条件优化; 酶活力
Keywords:
pectinase strain;  purification;  fermentation optimization for enzyme production;  enzyme activity
分类号:
Q93-33
DOI:
-
文献标志码:
AAdoi:103969/jissn16742869201204004
摘要:
为了从自然界中寻找一种果胶酶生产菌株，在果胶平板上经过筛选、复筛，分离出产果胶酶生产菌株，并将分离得到的菌株在摇瓶发酵培养、测定其酶活力，其发酵培养基的初始pH、最适温度、最适接种量及发酵周期进行初步研究.结果表明：经过筛选得到的产果胶酶菌株在发酵培养基的初始pH为7.0、培养温度为33 ℃、接种量为30%、发酵周期为56 h时，此菌株产酶的酶活力最高，优化后的条件大大提高了菌株的产酶能力.
Abstract:
A pectinase strain from the nature would was screened and rescreened on the culture dishes, and cultivated in the shake flask for fermentation. The enzyme activity of pectinase strain was also detected in the cultivation process. The initial pH value of fermentation medium, fermentation temperature，optimum innoculation amount and the fermentation period were studied preliminarily. The results show that the pectinase strain has the best enzyme activity when the initial pH value of fermentation medium is 7.0,the culture temperature is 33 ℃,the innoculation amount is 30% and fermentation period is 56 h.The optimized conditions can greatly improve the enzyme activity.

参考文献/References:

［1］张树政.酶制剂工业［M］.下册.北京:科学出版社,1984:233-236.
［2］王军,黄秀琴,王洁,等. 一株高产果胶酶菌的分离及其产酶条件的优化研究［J］.安徽农业科学,2009,37(36):17902-17904.
［3］裘纪莹,王未名,陈建爱，等.微生物果胶酶的研究进展［J］.中国食品添加剂,2010,1(4):238-241.
［4］沈萍,陈向东.微生物学实验［M］.北京:高等教育出版社,2007:28-34.
［5］毛炜坤,张振中,王向东,等.野生苎麻微生物脱胶优势菌的选育及其应用［J］.纺织学报,2010,31(11)：66-72.
［6］张浩森,缪静，余晓斌，等.果胶酶高产菌株的筛选及产酶条件的研究［J］.生物学杂志,2008,25(1):28-30.
［7］QU Er Jun, LI Wen Jian, LIU Yun Xiao, et al. Inducing and Screening of High Producing Pectinase Aspergillus niger Strain［J］. Agricultural Science and Technology,2010,11(4):22-29.
［8］Reid W W.The pectic enzymes of the fungus Byssochlamys fulva［J］. Biochem J,1952,50(3):289-292.
［9］Sivakumar D, Wijeratnam W R S, Wijesundera R L C. Effect of GRAS compounds on mycelial growth,pectic enzyme activity and disease severity of postharvest pathogens on rambutan［J］. Biomedical and Life Sciences,2001,29(2):135-141.
［10］Brien P, Zamani M. Production of pectic enzymes by barepatch isolates of Rhizoctonia solani AG 8［J］. Australasian Plant Pathology,2003,32(1):65-72.
［11］Neate S M, Cruickshank R H,  Rovira A D. Pectic enzyme patterns of Rhizoctonia solani isolates from agricultural soils in South Australia［J］. 1988,90(1):37-42.
［12］Volpi C, Janni M,  Lionetti V, et al. The Ectopic Expression of a Pectin Methyl Esterase Inhibitor Increases Pectin Methyl Esterification and Limits Fungal Diseases in Wheat［J］. Molecular Plant\|Microbe Interactions,2011,24(9):1012-1019.
［13］Conde E,  Moure A,  Dominguez H, et al. Purified Phenolics from Hydrothermal Treatments of Biomass: Ability To Protect Sunflower Bulk Oil and Model Food Emulsions from Oxidation［J］. J Agric Food Chem,2011,59(17): 9158-9165.
［14］Romero\|Cascales I, Ros\|García J M, López\|Roca J M, et al. The effect of a commercial pectolytic enzyme on grape skin cell wall degradation and colour evolution during the maceration process［J］. Food Chemistry,2012,130(3):626-631.

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