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[1]刘恋,黎莉*,方继德,等.枸杞子水提物提取工艺优化及其对酪氨酸酶活性影响[J].武汉工程大学学报,2011,(12):30-32.
　LIU Lian,LI Li,FANG Ji de,et al.Optimization of extraction technology and effect on tyrosinase activityof water extracts from Lycium barbarum fruit[J].Journal of Wuhan Institute of Technology,2011,(12):30-32.
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枸杞子水提物提取工艺优化及其对酪氨酸酶活性影响(/HTML)
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《武汉工程大学学报》[ISSN:1674-2869/CN:42-1779/TQ]

卷:
期数:
2011年12期
页码:
30-32
栏目:
化学与化学工程
出版日期:
2011-12-30

文章信息/Info

Title:
Optimization of extraction technology and effect on tyrosinase activity
of water extracts from Lycium barbarum fruit
文章编号:
16742869(2011)12003003
作者:
刘恋; 黎莉*; 方继德; 吕萌
武汉工程大学化工与制药学院,绿色化工过程教育部重点实验室,湖北 武汉 430074
Author(s):
LIU Lian; LI Li; FANG Jide; LU Meng
School of Chemical Engineering & Pharmacy,Wuhan Institute of Technology;
Key Laboratory for Green Chemical Process of Ministry of Education,Wuhan 430074,China
关键词:
枸杞; 水提物; 提取工艺; 酪氨酸酶
Keywords:
Lycium barbarum; water extracts; extraction technology; tyrosinase
分类号:
R284.2
DOI:
-
文献标志码:
AAdoi:10.3969/j.issn.16742869.2011.12.007
摘要:
研究了枸杞子水提物的提取工艺及对酪氨酸酶活性的影响.通过正交实验优化枸杞子水提物的提取工艺，研究了料液比、提取时间、提取次数对枸杞子水提物中多糖质量分数的影响.采用蒽酮硫酸法比色测定多糖质量分数，并用体外酪氨酸酶氧化法测定枸杞子水提物对酪氨酸酶活性的影响.枸杞子水提物的最佳提取工艺为料液比120，提取时间30 min，提取次数3次，在此条件下测定枸杞子水提物的多糖质量分数为28.43%.枸杞子水提物对酪氨酸酶具有明显抑制作用，其半数抑制质量浓度IC50为0.73 mg/mL，阳性对照品熊果苷IC50为0.28 mg/mL.说明枸杞子水提物对酪氨酸酶的抑制作用与熊果苷相当.
Abstract:
The extraction technology of the water extracts from Lycium barbarum fruit was studied and the effect on tyrosinase activity was investigated.The materialliquid ratio,extraction time,extraction times were studied as 3 factors through orthogonal experiments.The content of Lycium barbarum polysaccharides was determined by phenolsulphoacid method,and the effect on tyrosinase activity was measured by the rate of oxidation of Ltyrosine.The optimum technological parameters were as follows:materialliquid ratio l20,extraction time 30 min,and extraction for 3 times.The extraction rate of Lycium barbarum polysaccharides reached 28.43% at the conditions of optimum extraction.The water extracts of Lycium barbarum fruit inhibited the activity of tyrosinase extremely,the IC50 is 0.73 mg/mL,while the IC50 of the known tyrosinase inhibitor arbutin is 0.28 mg/mL.The results showed that the water extracts from Lycium barbarum fruit express the same inhibitory effect on tyrosinase activity as arbutin.

参考文献/References:

［1］李时珍.本草纲目［M］.北京：人民卫生出版社,1975:14521455.
［2］王金章,王秀娟.枸杞子的化学成分和药理研究概况［J］.天津药学,1999,11(3):1416.
［3］丁大鹏,马文丽,郑文岭.酪氨酸酶抑制剂的研究进展［J］.实用医学杂志,2005,21(12):13641365.
［4］Wu S J,Ng L T,Lin C C.Antioxidant activities of some common ingredients of traditional chinese medicine,Angelica sinensis,Lycium barbarum and Poria cocos［J］.Phytother Res,2004,18(12):10081012.
［5］Luo Qiong,Cai Yi Zhong,Yan Jun,et al.Hypoglycemic and hypolipidemic effects and antioxidant activity of fruit extracts from Lycium barbarum［J］.Life Sciences,2004,76(2):137149.
［6］Cai Yi Zhong,Luo Qiong,Sun Mei,et al.Antioxidant activity and phenolic compounds of 112 traditional Chinese medicinal plants associated with anticancer［J］.Life Sciences,2004,74(6):21572184.
［7］邱敏芳，黎莉，方继德,等.番石榴多糖提取工艺的优化［J］.武汉工程大学学报,2011,33(1):2224.
［8］易剑平,毕雅静,宋秀荣,等.蒽酮一硫酸法测定构祀多糖质量分数的研究［J］.北京工业大学学报,2005,31(6):641646.
［9］蒋萌蒙,田呈瑞,王向军.双孢蘑菇中酪氨酸酶的特性［J］.江苏农业学报,2008,24(2):194198.
［10］魏永祥,商希礼.枸杞多糖的研究进展［J］.安徽农业科学,2010,38(11):58345836.

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