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[1]王宗春,罗丽萍,周延安,等.注射用磷酸肌酸钠中细菌内毒素的检测[J].武汉工程大学学报,2010,(11):28-30.[doi:10.3969/j.issn.16742869.2010.11.008]
　WANG Zong chun,LUO Li ping,ZHOU Yan an,et al.Detection of bacterial endotoxin in creatine phosphate sodium for injection[J].Journal of Wuhan Institute of Technology,2010,(11):28-30.[doi:10.3969/j.issn.16742869.2010.11.008]
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《武汉工程大学学报》[ISSN:1674-2869/CN:42-1779/TQ]

卷:
期数:
2010年11期
页码:
28-30
栏目:
化学与化学工程
出版日期:
2010-11-30

文章信息/Info

Title:
Detection of bacterial endotoxin in creatine
phosphate sodium for injection
文章编号:
16742869(2010)11002803
作者:
王宗春; 罗丽萍; 周延安; 宋金春
武汉大学人民医院药学部,湖北 武汉 430060
Author(s):
WANG Zongchun;  LUO Liping;  ZHOU Yanan;  SONG Jinchun
Department of Pharmacy, Renmin Hospital of Wuhan University, Wuhan 430060,China
关键词:
细菌内毒素; 鲎试剂; 注射用磷酸肌酸钠
Keywords:
bacterial endotoxin;  tachypleus amebocyte lysate;  creatine phosphate sodium for injection
分类号:
R927.12
DOI:
10.3969/j.issn.16742869.2010.11.008
文献标志码:
A
摘要:
考察采用鲎试剂方法检测注射用磷酸肌酸钠中细菌内毒素的可行性.选择灵敏度为0.5 EU/mL的鲎试剂,通过对不同浓度的注射用磷酸肌酸钠供试品溶液进行细菌内毒素与鲎试剂之间凝集反应的干扰试验,结果显示：浓度为2.0 mg/mL的供试品溶液对鲎试剂与细菌内毒素之间的凝集反应既无抑制作用,也无增强作用。因此,使用鲎试剂方法检测注射用磷酸肌酸钠中细菌内毒素具有简便、快捷、可靠等优点.
Abstract:
The feasibility for detecting the bacterial endotoxin in creatine phosphate sodium for injection via tachypleus amebocyte lysate test was evaluated. Using the tachypleus amebocyte lysate with sensitivity of 0.5 EU/mL, the experiment was carried out to study the interference effect of samples concentration on the congregating reactivity between tachypleus amebocyte lysate and bacterial endotoxin. The results showed that samples had no inhibitive or enhanced effect on the congregating reactivity when samples concentration was 2.0 mg/mL, indicating that the method of tachypleus amebocyte lysate test for detecting the bacterial endotoxin in creatine phosphate sodium for injection is simple, rapid and reliable.

参考文献/References:

［1］国家药典委员会.中华人民共和国药典二部［M］.北京:中国医药科技出版社,2010:附录99102.
［2］中国药品生物制品检定所.中国药品检验标准操作规范［M］.北京:中国医药科技出版社,2005:287302.
［3］施震,尹银嘉,王宗春.鲎试剂方法检测注射用盐酸头孢吡肟中细菌内毒素［J］.中国抗生素杂志,2003,28(2):121123.
［4］陈丹丹,黄清泉,王春仁.医用聚乙二醇小檗碱液的细菌内毒素检测［J］.药物分析杂志,2009,29(10):17621764.
［5］张德波,仲华.注射用水溶性维生素细菌内毒素检查法质量标准研究［J］.中国药品标准,2008,9(3):198201.
［6］张义伟.细菌内毒素检查的几点体会［J］.中国实用医药,2010,5(3):8687.

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备注/Memo

备注/Memo:
张瑞收稿日期：20100727作者简介：王宗春(1967)，男，江西宜春人，副主任药师，博士.研究方向：药物制剂及分析.
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